Matches included a number of genes related to insecticide resistance. We also compiled four digital gene expression libraries to investigate the expression profiles of genes at different developmental stages. These assembled, annotated transcriptome sequences and gene expression profiles extend the genomic resources available for researchers studying B. dorsalis and may provide a fast approach to identifying genes involved in development and insecticide resistance. According to the blast results in GenBank, only 881 nucleotide sequences of B. dorsalis had been previously Foretinib abmole bioscience submitted. One of the purposes of this is to find an efficient way to control this pest. In recent years, in China, geographically widespread populations of B. dorsalis have developed high levels of resistance to commonly used insecticides, such as trichlorphon, bcypermethrin, and avermectin. However, the molecular mechanisms of resistance are still unknown and the main obstacle to further research is the limited amount of genetic information. To assist research on insecticide resistance, we surveyed our transcriptome database and identified the most important enzymes related to the metabolism of insecticides or genes encoding proteins that are the targets of insecticides. The P450s are a major family of enzymes involved in detoxification and metabolism. Before our study, only seven P450 sequences of B. dorsalis were available in GenBank. In this study, 51 additional unique sequences encoding P450 genes were selected and submitted. These genes belong to several families; most are members of the CYP4, CYP6, and CYP12 families, according to phylogenetic analysis and BLAST results. GSTs play an important role in phase II detoxification of the hydrophobic toxic compounds found in insecticides. They are thought to be mainly involved in the detoxification of organophosphates, pyrethroids, and organochlorines. In D. melanogaster, 37 GSTs genes have been identified. However, none had been reported for B. dorsalis. In insects, mutations occurring in CarEs genes could potentially increase the rate of insecticide hydrolysis, such as that of organophosphates, or it could decrease activity towards generic substrates, such as naphthyl acetate. Although many point mutations related to insecticide resistance have been found in insects no CarEs genetic information had been reported for B. dorsalis. In the transcriptome database, 12 CarEs gene sequences were discovered and submitted. In this way, our work provides a basis for understanding mechanisms of insecticide resistance and could greatly improve future studies of this pest at the molecular level. However, it should be pointed out that although a large number of potentially interesting genes were obtained from the transcriptome data, most of them were partial sequences of specific genes and some of the unigenes were allelic variants or located on different part of the same gene. Due to short size or poor alignment, some sequences were excluded from analysis. In this way, when using this type of data to find genes of interest, particular attention should be paid to identifying each unigene to confirm that it is unique. To solve this problem, RACE technology is the preferred choice for future classification and obtaining the full length of these genes.