The flow cytometry analysis result showed that SB treatment caused decrease in platelet size and the scanning electron microscopic examination showed that SB treatment caused change in platelet shape. The change in cytoskeleton of platelets might be related to change in intracellular Ca level and subsequent regulation of proteins including copine I, INCB28060 c-Met inhibitor coronin-1B, LIM domain protein CLP-36 and cytoplasmic dynein intermediate chain 2C. Copine I belongs to a ubiquitous family of calcium-dependent, membrane-binding proteins. In response to changes in intracellular Ca, copines might regulate the activities and localization of their target proteins such as MEK1. Previous reports suggested that integrin a2b1 binding caused reorganization of cytoskeleton in platelets through MAPK pathway. Coronins are a conserved family of WD repeat-containing, actin-binding proteins involved in modulating actin dynamics and cell motility. SB-induced increase in coronin-1 B in platelets was showed to be dependent on integrin a2b1 binding in the present study. LIM domain protein CLP-36 is a cytoskeletal and anchoring protein that has been suggested to be closely related to the plasma membrane Ca-ATPase, which plays an essential role in maintaining cytosolic Ca level in platelets. Cytoplasmic dynein intermediate chain 2C is a component of the complex microtubule-based system of motor proteins in platelet. In all, the present study identified 20 target-related proteins of SB in platelets through proteomic analysis. Signal network of SB from binding to its direct target integrin a2b1 was predicted and then certified. Binding of SB to integrin a2b1 caused change in level of intracellular Ca, level of cytoskeleton-related proteins such as coronin-1 B and cytoskeleton structure of platelets. Signal cascades after SB binding to integrin a2b1 might be important basis for the inhibitive effect of SB on platelet adhesion and aggregation. To date, this study is the first to employ the proteomic technique to search globally for the proteins influenced in platelets by SB. The result of proteomic analysis and the suggested signal cascades network provided new hints for the study of the mechanism of SB on platelets. Non-small cell lung cancer is a common, largely incurable cancer. In the early disease setting, surgical resection is the standard of care and the benefit of adjuvant chemotherapy is limited to 5–15% improvement in survival in stage II but not stage I disease. However relapse is frequent in stage I cancer with 37% of patients dying within five years, therefore there is a need to identify reliable biomarkers of poor outcome in order to target individuals for whom adjuvant or novel targeted therapy may improve survival.