NSCs were utilized to overexpress NT-3 to affect the survival and differentiation of surrounding cells. However, gene transduction is limited by various problems, including concerns about the safety and efficiency of gene transfection and the potential adverse effects of exogenous gene expression. In contrast, PCLA-SF has several advantages for NT-3 delivery. First, SF adsorption and b-sheet formation is a simple method to immobilize growth factors. Multiple growth factors can also be immobilized simultaneously with SF coating, which synergistically improve functional recovery. To the best of our knowledge, this is the first time a multi-parameter analysis of cellular growth and motility from quantitative phase images during epithelial wound healing has been performed. In this study, we prove DHM to enable continuous, stain-free monitoring of intestinal epithelial wound healing in vitro and to provide simultaneous quantification of key cellular characteristics such as cell volume, cell thickness, dry mass and cell density which may help to characterize therapeutic effects of potential drug candidates. Proliferation and migration are two major steps required for successful wound closure following ulceration and inflammation and numerous agents. Preclinical evaluation of potential drug candidates in vitro is traditionally performed by the use of mechanically induced wounds and healing assessed by the number of cells beyond the wound edge. However, this experimental approach is limited due to its inability to discriminate migrating from proliferating cells and the necessity of staining to identify cell borders, which excludes repetitive measurements of migrating cells. While acceleration of epithelial migration benefits wound closure, enhanced proliferation may be associated with adverse side effects such as malignant transformation and morphological changes. In our hands, quantitative DHM phase contrast images in combination with time lapse analysis allowed quantification of the cell-covered area as well as accurate identification of proliferation cells by quantification of cellular dry mass and morphology.