This analysis identifies downstream targets of LH signaling that may be important for ovulation. Two interesting differences between ovulation and atresia were the expression networks for activin and inhibin. A gene network for activin was significantly increase 96% at ovulation while a gene network for inhibin B was significantly decreased 136% during atresia. As with the GSEA analysis, red indicates an increase in expression while green indicates a decrease in expression levels. The major cell processes that were significantly associated with the genes involved in the network were also included and represent many of the cell signaling cascades identified by the GSEA. For the cell processes that were mapped to the two gene expression networks, the processes of DNA replication, lipid transport, and extracellular protein complexes were differentially impacted between ovulation and atresia. Energy storage and metabolism were increased in both networks. In the inhibin B network, cell respiration and reactive oxygen species were increased while extracellular protein networks were depressed in the trajectory to atresia, which corresponds to the GSEA analysis. The expression patterns of mPR-alpha and ghr1 were corroborated by both real-time and microarray data in this study, and each approach revealed that there were higher levels of the transcripts at earlier stages of ovary development. The transcripts lhr and fshr were not present on the microarray but were examined with real-time PCR. Transcript expression patterns for genes such as star and esrba have also been previously described in the LMB ovary and corroborate the ovarian transcriptomics data presented here. For example, the microarray data suggest that star is increased in expression towards oocyte maturation and that esrba is also increased in expression at OV and at AT. In fish, this gene network may be functioning to maintain or enhance neuronal inputs during final oocyte maturation. In contrast, STATs play key roles in growth factor-mediated intracellular signal transduction, proliferation, inflammation, and apoptosis. In the rat ovary, STAT5b and STAT3 signaling pathways show temporal expression patterns during folliculogenesis and luteinization and these pathways are active at different periods to regulate gene expression. STAT5 activates a number of transcription factors and during AT, there may no longer be a need for cell regulation and active DNA transcription. Gene networks that require more discussion include those controlled by activin/inhibin. Both activin and inhibin are cytokines that are found in the CNS as well as in peripheral tissues. Over 4 million Americans and 65 million people worldwide have glaucoma, making it the leading cause of blindness in the US and the second leading cause of blindness worldwide. Although available therapies delay disease progression, protection remains incomplete and vision loss due to Rapamycin glaucoma cannot be regained, highlighting the need for novel therapeutic approaches and drug targets. In primary open-angle glaucoma, one of the 
most common glaucoma subtypes, there is variable elevation of intraocular pressure associated with impaired aqueous outflow that occurs Talazoparib despite normal anterior segment anatomy and an open iridocorneal angle.