Unlike serine and threonine Sennoside-D phosphorylation modifications, the rules of consensus do not work well with tyrosine phosphorylation, and programs based on algorithms to Chrysophanol-8-O-beta-D-glucopyranoside predict tyrosine phosphorylation have not matched experimental outcomes. Hence a comprehensive high-throughput effort focused on generating tyrosine phosphorylation profiles will add to the knowledge base used to construct robust algorithms based on large datasets. Here we report a phosphor-proteome from Drosophila exclusively focused on tyrosine phosphorylation events under insulin and EGF signaling pathways. We also present the salient features of the Drosophila proteome architecture and the comparative proteomic analysis for conserved tyrosine phosphorylation events on human proteins. About one-fourth of the phosphoproteins found in this study have no known molecular or biological function, but about half of these have motifs suggestive of their molecular function. In the context of Drosophila development, phosphoproteins found in this study are involved in early embryogenesis, cellularization, early and late blastulation, gastrulation, patterning and cell migration. Proteins involved in major organogenesis pathways such as heart and muscle development, tubulogenesis, dorsal vessel, CNS development and reproductive system and down-regulation of RTKs are also phosphorylated. A detailed illustration of various organ systems, cellular processes and signaling pathways represented by the tyrosine phosphorproteome is illustrated in Figure 2. The tyrosine phosphopeptide profiles presented here represent the largest dataset reported in Drosophila to date. This dataset is unique because it highlights activated proteins upon activation of growth factor RTKs. Many of the novel phosphorylation events found in this study on proteins previously not known to be involved in RTK pathways, represent new signaling nodes that merit further validation.After the shoots developed adequate roots, they were transplanted into greenhouse. LS1, LS3, and the control mutants were identified when grown in the greenhouse.