These might include eliminating the use of sodium sulfite or establishing a level of acceptable risk that is consistent with other forms of indirect fishing related mortality.We urge caution when using this approach as we have no data on additive effects or the effects of other cure ingredients. It is likely that other components of some cures are as toxic but currently have little or no effect as they are Staurosporine present at lower levels. Any increase in the concentrations of these compounds as a result of decreasing the concentration of sodium sulfite may result in a similar problem. Similarly, there are no data on the sublethal effects of sulfite exposure in juvenile salmon. A number of studies have shown that exposure to sulfites may have sublethal effects in other vertebrates, including alteration of mineral balance and damage to renal cell membranes. We have no data to suggest that ingestion of sulfites alters the ability of juvenile salmon to osmoregulate or maintain proper ion balance. However, given that smolts may be exposed to sulfites prior to ocean entry this should be given some consideration. However, patient’s age, degree of anemia, serum creatinine and calcium levels, dehydrogenase activity as well as cytogenetic abnormalities have been described as additional prognostic factors. Plasma cell morphology has also been recognized as an independent prognostic factor for more than two decades. However, the recognition of prognostically relevant cell atypias and maturation of the neoplastic plasma cells is dependent on the morphologist’s expertise,LY2835219 thus leading to a considerable interobserver variability. On the other hand, experimental studies using computerized image analysis have shown that subvisible modifications of the chromatin architecture in myeloma cells are associated with important changes of cell physiology, such as acquired drug resistance. The quantitative analysis of the distribution pattern of chromatin and other nuclear components has been shown to be of prognostic importance in several neoplasias. Therefore, we would expect that chromatin texture features in myeloma cells might reveal characteristics related to the aggressiveness of the tumor. Scale-invariant self-similarity is an important feature of many biological structures.