In this study, the MCF7 cells treated with AbMole Nodakenin evodiamine at earlier time point showed a significant up-regulation in the expression of 
Nbk and Bax and leaded to the activation of caspase and apoptosis. On the other hand, the expressions of ERa and ERb were also downregulated after treatment of evodiamine in MCF-7 cells. This effect also decreased the proliferation of estrogen-dependent MCF-7 cells. In conclusion, the effects of evodiamine include the decrease of cell proliferation and up-regulation of apoptosis-related molecules, such as caspase 7, PARP, Nbk, and Bax in MCF-7 cells. These results suggest that evodiamine may in part mediate through ERinhibitory pathway to reduce breast cancer cell proliferation. Alternative splicing of the pre-mRNA is a powerful and versatile regulatory mechanism that allows the production of multiple protein variants from a single gene and affects the quantitative control of gene expression and the functional diversification of proteins. As the major enamel matrix protein contributing to tooth development, amelogenin has been demonstrated to play a crucial role in tooth enamel formation. Amelogenin has been identified in many species and its functions have been extensively studied in mammalian vertebrates, especially in mouse, rat, and bovine. It has been reported that mammalian amelogenin exhibits heterogeneity in tooth organs and that different isoforms play different roles during enamel formation. The heterogeneity of amelogenin is believed to be generated either from alternative splicing of amelogenin or posttranslational processing of the amelogenin protein. The mammalian amelogenin gene is commonly organized into 7 exons, except in rats and mice in which it contains two additional exons. Analysis of amelogenin gene genomic sequences revealed a piece of sequence homologous to exon 4 in mouse, rat, guinea pig, and deer mouse. This short piece of sequence is located immediately upstream of exon 8 and designated as exon 4b. However, exon 4b was never shown to be coding in any rodent species. A full-length amphibian and sauropsids amelogenin gene was reported to contain 6 exons, and the absence of exon 4 in lizard and frog genomic DNA sequence has been confirmed. However, a recent study has identified a novel amelogenin transcript in a reptile that contains 7 exons. This transcript variant contains a unique exon X located between exon 5 and 6. Although the amelogenin gene and its splicing forms have been identified and characterized in mammals, little is known about amelogenin alternative splicing in non-mammalian vertebrates, especially in lipidosaurs and amphibians. To explore amelogenin alternative splicing in amphibians, we selected a caudate amphibian, the salamander Plethodon cinereus, as an animal model and employed PCR and sequence analysis to identify the potential amelogenin splicing transcripts.