In particular, ChIP-seq data for BMAL1, ROR ��,�� and REV-ERB��,�� confirmed links between the ECCN and several cancer-related genes. Notably, two of these genes were shown to be involved in miRNA regulation. The mammalian circadian clock is an endogenous, time-generating system with the peculiarity of synchronizing and propagating time-cues to the entire organism. Its relevance in the time dependent regulation of biological processes has been shown at the organismal and cellular levels. As such, it is of no surprise that malfunctions of the circadian system were found to be associated to pathological phenotypes including obesity, sleep disorders and increasing incidence of cancer. The prospect of using individual patient-timing, based on the internal circadian clock, for therapy optimization is being explored with promising results. For instance, advances in chrono therapy have proven to be efficient in reducing toxicity and increasing Ronidazole efficacy yin some types of cancer, particularly colon cancer. A more detailed knowledge of the circadian network including the pathways it regulates is of major importance for the analysis on how time effects may be propagated and to determine the time-dependent action of certain drugs. In this work we setup to dissect such clock-regulated pathways and to analyse the extent of circadian regulation at the cellular level by expanding the core circadian network to its potential l target genes. We used human high-throughput transcript to me-datasets associated to text mining of biomedical literature, for denovoclock regulated gene discovery. Therefore, we used a hybrid methodology where to the expression correlation data we associated the text mining as an independent source of knowledge, GW843682X enabling us to find regulated genes and their connection to the ECCN with increased confidence. This allowed us to partially overcome the limitations of expression analysis in terms of network topology and to be able to generate a semi-regulatory network for the mammalian circadian clock. Still, we do not analysetissue-specificity issues which go beyond the scope of this work. Nevertheless, the circadian clock has been reported, in mammals, to be present in all cells so that the core network is expected to be very similar.