As it is not known whether these nanofiber-expanded stem cells are biologically active to be used clinically for the treatment of myocardial ischemia, we sought to investigate the functional role of these cells in a rat myocardial infarction model. Cell based therapeutic approaches may prevent deterioration of the myocardial function post infarction and may even reverse established heart failure. However, the original concepts regarding cell-based therapies have proven to be overly simplistic since generation and engrafment of new muscle alone is insufficient for the approach to be successful. It appears that nonmyogenic mechanisms are important for majority of the beneficial trophic effects observed during cell-based therapies. Therefore, elucidation of these specific mechanisms is essential to improve cell-based therapies. In the present study, we first expanded stem cells ex vivo on nanofiber-coated plates and then overexpressed VEGF and PDGF in these cells to enhance their vasculogenic potential. Previous studies of autologous BM-derived CD133+ cells injected either via intracoronary infusion or intramyocardial injection augmenting vasculogenesis in patients with myocardial ischemia prompted us to investigate the potential of genetically Phloridzin modified stem cells in ischemic heart disease. Furthermore, the limited availability of functional progenitor cell population in bone marrow and peripheral circulation and compromised potential of these cells in disease states and aged individuals has hindered the study of underlying mechanisms of successful cell-based therapy in these subjects. Our current findings thus provide the feasibility and effectiveness of overexpressing angiogenic growth factors on nanofiber expanded hematopoietic stem cells for the treatment of myocardial infarction. The overexpression of angiogenic factors not only promotes neovascularization in the ischemic heart tissues, but thereby significantly improves several parameters of cardiac function including fractional shortening, tissue velocity, wall motion score index, strain and strain rate. Accordingly, with improved heart function, animals Sarsasapogenin demonstrate better exercise capacity implying functional improvement.