The advantage of ‘omics’ technologies lies in their multiparametric nature probing a large array of indicators at the same time. For the application of ‘omics’ technologies to the understanding of drug action, it is essential that effects of drugs produce a sensitive response which can be reproduced with sufficiently high quality. Here we have shown that metabolic profiling of cancer cells can not only identify phenotypic differences between cell lines but can also sensitively detect and distinguish changes in metabolite concentrations induced by relatively short exposure to differing drugs. It is important to mention that all treatments caused a distinct reproducible profile which is observed in unsupervised PCA. The schematics shown in Figure 5 and Figure S3 depict the commonalities and differences in changes in metabolites when each of the cell lines was treated with combined MPA+BEZ. These figures powerfully illustrate that a common metabolic response underlies the perception of the drugs in independent AML cell lines with markedly differing constitutive metabolomes. For the functional relevance it is essential that two similar cell lines, K562 and KG1a, representing blockage at an early stage of maturation of the erythroid and myeloid lineages showed a similar metabolic response to the MPA+BEZ treatment whereas HL-60 cells, which are closer to the mature granulocyte stage showed a different overall response. HL-60 also showed a less pronounced combinatorial effect for combined MPA+BEZ treatment which was clearly distinguished from individual treatments in KG1a and K562. Here we report the design and diagnostic validation of an unbiased high-throughput sequencing method for the direct diagnosis of viral infections in clinical specimens. SAR131675 VEGFR/PDGFR inhibitor Patient samples were obtained during seasonal influenza virus infections and norovirus outbreaks from 2005 to 2007 in Osaka, Japan. cDNAs, as templates for the GS FLX platform, were prepared by random RTPCR using RNAs extracted from clinical samples. High-throughput sequencing yielded 15,298–32,335 sequences, of which 7–15,260 represented the targeted viral sequences. Another possible regulator of SNARE function is Sro7p and its redundant homologue SRO77. Sro7p and Sro77p have been implicated in secretion based on genetic studies demonstrating decreased secretion of invertase and an accumulation of secretory vesicles in the absence of SRO7 and SRO77. SRO7 was initially isolated as a high-copy suppressor of rho3 mutants, suggesting a role for Sro7p in maintenance of actin polarity. However, further studies have established that the primary role for Sro7p is in membrane fusion. First, Sro7p binds directly to Sec9p, and the interaction between Sro7p and SNAREs is essential for Sro7p function. Second, Sro7p is an effector of the Rab GTPase Sec4p, which has multiple functions during secretion, one of which occurs after vesicle transport to sites of secretion.